Micropatterned substrates coated with neuronal adhesion molecules for high-content study of synapse formation.
Nat Commun. 2013-08-12; 4(1):
DOI: 10.1038/ncomms3252
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1. Nat Commun. 2013;4:2252. doi: 10.1038/ncomms3252.
Micropatterned substrates coated with neuronal adhesion molecules for
high-content study of synapse formation.
Czöndör K(1), Garcia M, Argento A, Constals A, Breillat C, Tessier B, Thoumine O.
Author information:
(1)University of Bordeaux, Interdisciplinary Institute for Neuroscience, UMR
5297, F-33000 Bordeaux, France.
Studying the roles of different proteins and the mechanisms involved in
synaptogenesis is hindered by the complexity and heterogeneity of synapse types,
and by the spatial and temporal unpredictability of spontaneous synapse
formation. Here we demonstrate a robust and high-content method to induce
selectively presynaptic or postsynaptic structures at controlled locations.
Neurons are cultured on micropatterned substrates comprising arrays of
micron-scale dots coated with various synaptogenic adhesion molecules. When
plated on neurexin-1β-coated micropatterns, neurons expressing neuroligin-1
exhibit specific dendritic organization and selective recruitment of the
postsynaptic scaffolding molecule PSD-95. Furthermore, functional AMPA receptors
are trapped at neurexin-1β dots, as revealed by live-imaging experiments. In
contrast, neurons plated on SynCAM1-coated substrates exhibit strongly patterned
axons and selectively assemble functional presynapses. N-cadherin coating,
however, is not able to elicit synapses, indicating the specificity of our
system. This method opens the way to both fundamental and therapeutic studies of
various synaptic systems.
DOI: 10.1038/ncomms3252
PMID: 23934334 [Indexed for MEDLINE]