Micropatterned “adherent/repellent” glass surfaces for studying the spreading kinetics of individual red blood cells onto protein-decorated substrates
European Biophysics Journal. 2003-07-01; 32(4): 342-354
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1. Eur Biophys J. 2003 Jul;32(4):342-54. Epub 2003 Feb 19.
Micropatterned “adherent/repellent” glass surfaces for studying the spreading
kinetics of individual red blood cells onto protein-decorated substrates.
Cuvelier D(1), Rossier O, Bassereau P, Nassoy P.
(1)Unité Mixte de Recherches 168, Institut Curie, 26 rue d’Ulm, 75248 Cedex 05
We report in this paper two simple and effective methods to decorate glass
surfaces that enable protein micropatterning and subsequent spatially controlled
adhesion of cells. The first method combines simultaneously the potentialities of
two existing techniques, namely microcontact printing (muCP) and microfluidic
networks (muFN) to achieve dual protein patterning in a single step. The second
method is mainly based on the well-known property of poly(ethylene glycol) (PEG)
to resist against protein adsorption. Both approaches were used to produce
heterogeneous surfaces on which micron-size or submicronic streptavidin-coated
lines alternate with cell-repellent areas. We first describe the implementation
of the two methods and discuss the main pitfalls to avoid. Then, using these
templates, we have monitored the kinetics of attachment of individual
biotinylated (i.e. “attractant” towards streptavidin) red blood cells by directly
measuring the propagation velocity of the adhesion front. Depending on the
surface density of biotin, we found two distinct regimes, in agreement with
existing theoretical models.
PMID: 12851792 [Indexed for MEDLINE]