Microfluidic stickers for cell- and tissue-based assays in microchannels
Lab Chip. 2009-01-01; 9(7): 1011-1013
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1. Lab Chip. 2009 Apr 7;9(7):1011-3. doi: 10.1039/b819090a. Epub 2008 Dec 5.
Microfluidic stickers for cell- and tissue-based assays in microchannels.
Morel M(1), Bartolo D, Galas JC, Dahan M, Studer V.
(1)Laboratoire Kastler Brossel, CNRS UMR8552, Physics and Biology Department,
Ecole Normale Supérieure and Université Pierre et Marie Curie-Paris 6, France.
Difficulties in culturing cells inside microchannels is a major obstacle for the
wide use of microfluidic technology in cell biology. Here, we present a simple
and versatile method to interface closed microchannels with cellular and
multicellular systems. Our approach, based on microfluidic stickers which can
adhere to wet glass coverslips, eliminates the need to adapt cell culture
conditions to microchannels and greatly facilitates the methods required to
position cells into microcircuits. We demonstrate the simplicity and efficiency
of the method with HeLa cells, primary cultured neurons and Drosophila tissues.
PMID: 19294316 [Indexed for MEDLINE]