Mammalian cochlear outer hair cells density evaluated by means of an optical tweezer.
Biochemical and Biophysical Research Communications. 1993-10-01; 196(1): 363-368
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1. Biochem Biophys Res Commun. 1993 Oct 15;196(1):363-8.
Mammalian cochlear outer hair cells density evaluated by means of an optical
Laffon E(1), Dulon D, Pouligny B, Blanchet C, Aran JM.
(1)Laboratoire d’Audiologie Expérimentale, I.N.S.E.R.M. U. 229, Bordeaux, France.
We report on the first individual measurements of guinea pig’s cochlear outer
hair cells densities. Cells were isolated in vitro and manipulated with an
optical tweezer. They were levitated in an upward laser beam coaxially trapping
the cells. Then they were released by switching off the laser and let fall down
in upright position. Measuring their speed and using the Stokes’ law, we
calculated their mean density. In our experimental frame, the results suggest
that the density of the cellular body (between the basal nucleus and the apical
cuticular plate) remains quasi constant whatever the cells’ length. This implies
that density variation of the cellular body does not participate in an intrinsic
PMID: 8216313 [Indexed for MEDLINE]