Long-term evaluation of sensorimotor and mnesic behaviour following striatal NMDA-induced unilateral excitotoxic lesion in the mouse.
Behavioural Brain Research. 2007-03-28; 178(2): 235-243
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1. Behav Brain Res. 2007 Mar 28;178(2):235-43. Epub 2007 Jan 23.
Long-term evaluation of sensorimotor and mnesic behaviour following striatal
NMDA-induced unilateral excitotoxic lesion in the mouse.
Haelewyn B(1), Freret T, Pacary E, Schumann-Bard P, Boulouard M, Bernaudin M,
(1)CNRS UMR 6185 CYCERON, University of Caen Basse-Normandie, BP 5229, Bd H.
Becquerel, F-14074 Caen Cedex, France.
Excitotoxic lesion of the striatum provides a useful model for evaluating the
excitotoxic processes involved in neurological disorders, in particular stroke
diseases. The behavioural outcome after such injury is however poorly described.
We have therefore investigated the potential behavioural deficits induced by a
NMDA-induced excitotoxic unilateral lesion of the lateral part of the striatum,
by comparison with a PBS striatal injection (sham procedure), and non-operated
mice behaviour. Three groups of male adult Swiss mice were constituted:
unilateral NMDA (20 nmol striatal NMDA injection), sham (striatal PBS injection),
and control (healthy non-operated mice). From 14 to 29 days post-surgery,
sensorimotor and mnesic tests were performed in all groups. After euthanasia,
immunohistochemical stainings (NeuN and GFAP) were performed in order to assess
the size of the lesion. Straight runway and passive avoidance performances
revealed mild deficits related to the excitotoxic NMDA-induced lesion as compared
to the sham procedure. Moreover, accelerated rotarod and Morris water maze
acquisition performances also revealed deficits related to the surgery, i.e.
observed in sham-operated as compared to control mice. NeuN staining revealed no
striatal lesion in the sham and non-operated groups in contrast to the
NMDA-injected group in which the volume of infarcted striatum was 2.4+/-0.3mm3.
GFAP staining revealed a glial reaction in the lesioned striatum of NMDA animals
and at the PBS injection site in sham animals. These results suggest that
NMDA-induced excitotoxic lesion induces subtle long-term behavioural deficits in
mice. Moreover, this study shows the importance of the sham group to investigate
the behavioural deficits after excitotoxic lesion models in mice.
PMID: 17250902 [Indexed for MEDLINE]