Localization of corazonin in the nervous system of the cockroach Periplaneta americana
Cell Tissue Res. 1993-10-01; 274(1): 57-64
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1. Cell Tissue Res. 1993 Oct;274(1):57-64.
Localization of corazonin in the nervous system of the cockroach Periplaneta
Veenstra JA(1), Davis NT.
(1)Department of Entomology, University of Arizona, Tucson 85721.
Antisera raised to the cardioactive peptide corazonin were used to localize
immunoreactive cells in the nervous system of the American cockroach. Sera
obtained after the seventh booster injection were sufficiently specific to be
used for immunocytology. They recognized a subset of 10 lateral neurosecretory
cells in the protocerebrum that project to, and arborize and terminate in the
ipsilateral corpus cardiacum. They also reacted with bilateral neurons in each of
the thoracic and abdominal neuromeres, a single dorsal unpaired median neuron in
the suboesophageal ganglion, an interneuron in each optic lobe, and other neurons
at the base of the optic lobe, in the tritocerebrum and deutocerebrum. The
presence of corazonin in the abdominal neurons and the lateral neurosecretory
cells was confirmed by HPLC fractionation of extracts of the abdominal ganglia,
brains and retrocerebral complexes, followed by determination of corazonin by
ELISA, which revealed in each tissue a single immunoreactive peak co-eluting with
corazonin in two different HPLC systems. Antisera obtained after the first three
booster injections recognized a large number of neuroendocrine cells and neurons
in the brain and the abdominal nerve cord. However, the sera from the two rabbits
reacted largely with different cells, indicating that the majority of this
immunoreactivity was due to cross-reactivity. These results indicate that the
production of highly specific antisera to some neuropeptides may require a
considerable number of booster injections.
PMID: 8242711 [Indexed for MEDLINE]