Leucine supplementation modulates fuel substrates utilization and glucose metabolism in previously obese mice.
Obesity. 2013-09-05; 22(3): 713-720
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1. Obesity (Silver Spring). 2014 Mar;22(3):713-20. doi: 10.1002/oby.20578. Epub 2013
Leucine supplementation modulates fuel substrates utilization and glucose
metabolism in previously obese mice.
Binder E(1), Bermúdez-Silva FJ, Elie M, Leste-Lasserre T, Belluomo I, Clark S,
Duchampt A, Mithieux G, Cota D.
(1)NeuroCentre Magendie, INSERM, Physiopathologie de la Plasticité Neuronale,
U862, Bordeaux, France; NeuroCentre Magendie, Physiopathologie de la Plasticité
Neuronale, Université de Bordeaux, U862, Bordeaux, France.
OBJECTIVE: High-protein diets favor weight loss and its maintenance. Whether
these effects might be recapitulated by certain amino acids is unknown.
Therefore, the impact of leucine supplementation on energy balance and associated
metabolic changes in diet-induced obese (DIO) mice during and after weight loss
METHODS: DIO C57BL/6J mice were fed a normocaloric diet to induce weight loss
while receiving or not the amino acid leucine in drinking water. Body weight,
food intake, body composition, energy expenditure, glucose tolerance, insulin,
and leptin sensitivity were evaluated. Q-PCR analysis was performed on muscle,
brown and white adipose tissues.
RESULTS: DIO mice decreased body weight and fat mass in response to chow, but
supplementation with leucine did not affect these parameters. During weight
maintenance, mice supplemented with leucine had improved glucose tolerance,
increased leptin sensitivity, and lower respiratory quotient. The latter was
associated with changes in the expression of several genes modulating fatty acid
metabolism and mitochondrial activity in the epididymal white and the brown
adipose tissues, but not muscle.
CONCLUSIONS: Leucine supplementation might represent an adjuvant beneficial
nutritional therapy during weight loss and maintenance, because it improves lipid
and glucose metabolism and restores leptin sensitivity in previously obese
Copyright © 2013 The Obesity Society.
PMID: 23894080 [Indexed for MEDLINE]