Large-scale, high-density (up to 512 channels) recording of local circuits in behaving animals.

Antal Berényi, Zoltán Somogyvári, Anett J. Nagy, Lisa Roux, John D. Long, Shigeyoshi Fujisawa, Eran Stark, Anthony Leonardo, Timothy D. Harris, György Buzsáki
Journal of Neurophysiology. 2014-03-01; 111(5): 1132-1149
DOI: 10.1152/jn.00785.2013

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1. J Neurophysiol. 2014 Mar;111(5):1132-49. doi: 10.1152/jn.00785.2013. Epub 2013
Dec 18.

Large-scale, high-density (up to 512 channels) recording of local circuits in
behaving animals.

Berényi A(1), Somogyvári Z, Nagy AJ, Roux L, Long JD, Fujisawa S, Stark E,
Leonardo A, Harris TD, Buzsáki G.

Author information:
(1)New York University Neuroscience Institute, School of Medicine, New York
University, New York, New York;

Monitoring representative fractions of neurons from multiple brain circuits in
behaving animals is necessary for understanding neuronal computation. Here, we
describe a system that allows high-channel-count recordings from a small volume
of neuronal tissue using a lightweight signal multiplexing headstage that permits
free behavior of small rodents. The system integrates multishank, high-density
recording silicon probes, ultraflexible interconnects, and a miniaturized
microdrive. These improvements allowed for simultaneous recordings of local field
potentials and unit activity from hundreds of sites without confining free
movements of the animal. The advantages of large-scale recordings are illustrated
by determining the electroanatomic boundaries of layers and regions in the
hippocampus and neocortex and constructing a circuit diagram of functional
connections among neurons in real anatomic space. These methods will allow the
investigation of circuit operations and behavior-dependent interregional
interactions for testing hypotheses of neural networks and brain function.

DOI: 10.1152/jn.00785.2013
PMCID: PMC3949233
PMID: 24353300 [Indexed for MEDLINE]

Auteurs Bordeaux Neurocampus