Intrapallidal injection of 6-hydroxydopamine induced changes in dopamine innervation and neuronal activity of globus pallidus
Neuroscience. 2009-12-01; 164(2): 588-596
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1. Neuroscience. 2009 Dec 1;164(2):588-96. doi:
10.1016/j.neuroscience.2009.07.034. Epub 2009 Jul 21.
Intrapallidal injection of 6-hydroxydopamine induced changes in dopamine
innervation and neuronal activity of globus pallidus.
Bouali-Benazzouz R(1), Tai CH, Chetrit J, Benazzouz A.
(1)Université de Bordeaux, 146 Rue Léo-Saignat, 33076 Bordeaux Cedex, France.
The globus pallidus (GP) plays an important role in basal ganglia circuitry. In
contrast to the well-characterized actions of dopamine on striatal neurons, the
functional role of the dopamine innervation of GP is still not clearly
determined. The present study aimed to investigate the effects of intrapallidal
injection of 6-hydroxydopamine (6-OHDA) on rotational behavior induced by
apomorphine, on the loss of dopamine cell bodies in the substantia nigra pars
compacta (SNc) and fibers in the GP and striatum and on in vivo
extracellularly-recorded GP neurons in the rat. Injection of 6-OHDA into GP
induced severe loss of tyrosine hydroxylase-immunoreactive (TH-IR) fibers in GP
(-85%) with a reduction in the number of TH-IR cell bodies in the SNc (-52%) and
fibers in the striatum (-50%). S.c. injection of apomorphine in these rats
induced a moderate number of turns (26+/-6 turns/5 min). Electrophysiological
recordings show that 6-OHDA injection in GP induced a significant decrease of
the firing rate of GP neurons (16.02+/-1.11 versus 24.14+/-1.58 spikes/sec in
control animals and 22.83+/-1.28 in sham animals, one-way ANOVA, P