Inhibitory effect of the anorexic compound oleoylethanolamide on gastric emptying in control and overweight mice
J Mol Med. 2008-02-16; 86(4): 413-422
DOI: 10.1007/s00109-008-0305-7
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1. J Mol Med (Berl). 2008 Apr;86(4):413-22. doi: 10.1007/s00109-008-0305-7. Epub
2008 Feb 16.
Inhibitory effect of the anorexic compound oleoylethanolamide on gastric
emptying in control and overweight mice.
Aviello G(1), Matias I, Capasso R, Petrosino S, Borrelli F, Orlando P, Romano B,
Capasso F, Di Marzo V, Izzo AA.
Author information:
(1)Endocannabinoid Research Group, Department of Experimental Pharmacology,
University of Naples Federico II, Naples, Italy.
Gastric emptying regulates food intake. Oleoylethanolamide (OEA), an endogenous
acylethanolamide chemically related to the endocannabinoid anandamide, inhibits
food intake, but its effect on gastric emptying is unknown. Here, we
investigated the effect and the role of OEA on gastric emptying in mice fed
either a standard (STD) or a high-fat diet (HFD) for 14 weeks. Gastric emptying
was reduced by OEA, but not by its saturated analog, palmitoylethanolamide. The
effect of OEA was unaffected by rimonabant (cannabinoid CB1 receptor
antagonist), SR144528 (cannabinoid CB2 receptor antagonist),
5′-iodoresiniferatoxin (transient receptor potential vanilloid type 1
antagonist), or MK886 (peroxisome proliferator-activated receptor-alpha)
antagonist. Compared to STD mice, HFD mice showed delayed gastric emptying and
higher levels of gastric OEA. HFD-induced increase in OEA levels was accompanied
by increased expression of the OEA-synthesizing enzyme
N-acyl-phosphatidylethanolamine-selective phospholipase D and decreased
expression of the OEA-degrading enzyme fatty acid amide hydrolase. These results
might suggest that elevation of gastric OEA could possibly contribute to the
delayed gastric emptying observed in HFD-fed animals. HFD regulates OEA levels
in the stomach through an increase of its biosynthesis and a decrease of its
enzymatic degradation. The inhibitory effect of OEA on gastric emptying here
observed might underlie part of the anorexic effects of this compound previously
reported.
DOI: 10.1007/s00109-008-0305-7
PMID: 18278475 [Indexed for MEDLINE]