In vivo validation of a new portable stimulator for chronic deep brain stimulation in freely moving rats
Journal of Neuroscience Methods. 2020-03-01; 333: 108577
DOI: 10.1016/j.jneumeth.2019.108577
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Tibar H(1), Naudet F(1), Kölbl F(2), Ribot B(1), Faggiani E(1), N’Kaoua G(2), Renaud S(2), Lewis N(2), Benazzouz A(3).
Author information:
(1)Univ. de Bordeaux, Institut des Maladies Neurodégénératives, UMR 5293,
F-33000, Bordeaux, France; CNRS, Institut des Maladies Neurodégénératives, UMR
5293, F-33000, Bordeaux, France.
(2)Univ. de Bordeaux, Department of Bioelectronic, Laboratory IMS-CNRS UMR 5218,
F-33000, Bordeaux, France.
(3)Univ. de Bordeaux, Institut des Maladies Neurodégénératives, UMR 5293,
F-33000, Bordeaux, France; CNRS, Institut des Maladies Neurodégénératives, UMR
5293, F-33000, Bordeaux, France. Electronic address:
.
BACKGROUND: Deep brain stimulation (DBS) of the subthalamic nucleus (STN) is
considered as a gold standard therapy for the alleviation of motor symptoms in
Parkinson’s disease (PD). This success paved the way to its application for
other neurological and psychiatric disorders. In this context, we aimed to
develop a rodent-specific stimulator with characteristics similar to those used
in patients.
NEW METHOD: We designed a stimulator that can be connected to an electrode
container with options for bilateral or unilateral stimulation selection and
offers a wide range of frequencies, pulse widths and intensities, constant
current, biphasic current-control and charge balancing. Dedicated software was
developed to program these parameters and the device was tested on a bilateral
6-hydroxydopamine (6-OHDA) rat model of PD.
RESULTS: The equipment was well tolerated by the animals with a good general
welfare. STN stimulation (130 Hz frequency, 0.06 ms pulse width, 150 μA average
intensity) improved the motor deficits induced by 6-OHDA as it significantly
increased the number of movements compared to the values obtained in the same
animals without STN stimulation. Furthermore, it restored motor coordination by
significantly increasing the time spent on the rotarod bar.
CONCLUSION: We successfully developed and validated a new portable and
programmable stimulator for freely moving rats that delivers a large range of
stimulation parameters using bilateral biphasic current-control and charge
balancing to maximize tissue safety. This device can be used to test deep brain
stimulation in different animal models of human brain diseases.
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