In utero electroporation to study mouse brain development.

1. Methods Mol Biol. 2014;1082:285-93. doi: 10.1007/978-1-62703-655-9_19.

In utero electroporation to study mouse brain development.

Pacary E(1), Guillemot F.

Author information:
(1)Neurocentre Magendie, Physiopathologie de la Plasticité Neuronale, U862,
Institut National de la Santé et de la Recherche Médicale, Université de
Bordeaux, Bordeaux, France.

In utero electroporation is a rapid and powerful technique to study the
development of many brain regions. This approach presents several advantages over
other methods to study specific steps of brain development in vivo, from
proliferation to synaptic integration. Here, we describe in detail the individual
steps necessary to carry out the technique. We also highlight the variations that
can be implemented to target different cerebral structures and to study specific
steps of development.

DOI: 10.1007/978-1-62703-655-9_19
PMID: 24048941 [Indexed for MEDLINE]

Auteurs Bordeaux Neurocampus