Immunohistological localization of regulatory peptides in the midgut of the female mosquito Aedes aegypti
Histochem Cell Biol. 1995-11-01; 104(5): 337-347
DOI: 10.1007/BF01458127
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1. Histochem Cell Biol. 1995 Nov;104(5):337-47.
Immunohistological localization of regulatory peptides in the midgut of the
female mosquito Aedes aegypti.
Veenstra JA(1), Lau GW, Agricola HJ, Petzel DH.
Author information:
(1)Department of Entomology, University of Arizona 85721, USA.
The midgut of the female mosquito Aedes aegypti was studied immunohistologically
with antisera to various regulatory peptides. Endocrine cells immunoreactive with
antisera to perisulfakinin, RFamide, bovine pancreatic polypeptide, urotensin 1,
locustatachykinin 2 and allatostatins A1 and B2 were found in the midgut.
Perisulfakinin, RFamide and bovine pancreatic polypeptide all react with the
same, about 500 endocrine cells, which were evenly distributed throughout the
posterior midgut, with the exception of its most frontal and caudal regions. In
addition, these antisera recognized three to five neurons in each ingluvial
ganglion and their axons, which ran longitudinally over the anterior midgut, as
well as axons innervating the pyloric sphincter. The latter axons appear to be
derived from neurons located in the abdominal ganglia. Antisera to two different
allatostatins recognized about 70 endocrine cells in the most caudal area of the
posterior midgut and axons in the anterior midgut whose cell bodies were probably
located in either the brain or the frontal ganglion. Antiserum to
locustatachykinin 2 recognized endocrine cells present in the anterior midgut and
the most frontal part of the posterior midgut, as well as about 50 cells in the
most caudal region of the posterior midgut. Urotensin 1 immunoreactivity was
found in endocrine cells in the same region as the perisulfakinin-immunoreactive
cells, but no urotensin-immunoreactive axons were found in the midgut. Double
labeling experiments showed that the urotensin and perisulfakinin
immunoreactivities were located in different cells. Such experiments also showed
that the locustatachykinin and allatostatin immunoreactivities in the most caudal
area of the posterior midgut were present in different cells. No immunoreactivity
was found in the mosquito midgut when using antisera to corazonin, allatropin or
leucokinin IV. Since these peptides have either been isolated from, or can
reasonably be expected to be present in mosquitoes, it was concluded that these
peptides are not present in the mosquito midgut.
DOI: 10.1007/BF01458127
PMID: 8574883 [Indexed for MEDLINE]