Immunocytochemical localization of peptidergic cells in the neuro-endocrine system of the Colorado potato beetle, Leptinotarsa decemlineata, with antisera against vasopressin, vasotocin and oxytocin

J. A. Veenstra, H. M. Romberg-Privee, H. Schooneveld
Histochemistry. 1984-01-01; 81(1): 29-34
DOI: 10.1007/BF00495397

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1. Histochemistry. 1984;81(1):29-34.

Immunocytochemical localization of peptidergic cells in the neuro-endocrine
system of the Colorado potato beetle, Leptinotarsa decemlineata, with antisera
against vasopressin, vasotocin and oxytocin.

Veenstra JA, Romberg-Privee HM, Schooneveld H.

Antisera against vasopressin, vasotocin, oxytocin, neurophysin-1 and
neurophysin-2 were used to investigate immunocytochemically the presence of
neurons containing substances antigenically related to these peptides in the
nervous system of the Colorado potato beetle. Ten different antisera were used,
four against vasopressin, three against oxytocin and one against vasotocin,
neurophysin-1, and neurophysin-2. Immunoreactivity was shown by all antisera
except those against the neurophysins. The vasopressin antisera all gave
different results. One antiserum revealed only a single neuron pair, whereas
others revealed in addition one or two other different cell groups. The oxytocin
antisera likewise revealed different neurons. The fixation procedure influenced
the outcome of the immunocytochemical reaction. Immunoreactivity as revealed by
vasopressin, vasotocin and oxytocin antisera is often co-localized in the same
neurons; solid phase adsorptions showed that this is due to cross-reactivity of
the antisera. Some of the immunoreactive neurons are identical to those recently
described to contain a bovine pancreatic polypeptide/FMRFamide-like peptide. This
co-localization is probably not due to a cross-reaction. These findings indicate
the presence of several vasopressin-like and oxytocin-like substances which in
the Colorado potato beetle all have a different degree of immunocytochemical
resemblance to vasopressin and oxytocin.

DOI: 10.1007/BF00495397
PMID: 6469720 [Indexed for MEDLINE]

Auteurs Bordeaux Neurocampus