Imaging the adenovirus infection cycle

Noémie Pied, Harald Wodrich
FEBS Lett. 2019-12-01; 593(24): 3419-3448
DOI: 10.1002/1873-3468.13690

PubMed

Pied N(1), Wodrich H(1).

Author information:
(1)CNRS UMR 5234, Microbiologie Fondamentale et Pathogénicité, Université de
Bordeaux, France.

Incoming adenoviruses seize control of cytosolic transport mechanisms to
relocate their genome from the cell periphery to specialized sites in the
nucleoplasm. The nucleus is the site for viral gene expression, genome
replication, and the production of progeny for the next round of infection. By
taking control of the cell, adenoviruses also suppress cell-autonomous immunity
responses. To succeed in their production cycle, adenoviruses rely on
well-coordinated steps, facilitated by interactions between viral proteins and
cellular factors. Interactions between virus and host can impose remarkable
morphological changes in the infected cell. Imaging adenoviruses has
tremendously influenced how we delineate individual steps in the viral life
cycle, because it allowed the development of specific optical markers to label
these morphological changes in space and time. As technology advances,
innovative imaging techniques and novel tools for specimen labeling keep
uncovering previously unseen facets of adenovirus biology emphasizing why
imaging adenoviruses is as attractive today as it was in the past. This review
will summarize past achievements and present developments in adenovirus imaging
centered on fluorescence microscopy approaches.