How has dental pulp stem cells isolation been conducted? A scoping review

Camila Perelló Ferrúa, Eduarda Gervini Zampieri Centeno, Laísa Camerini da Rosa, Cainá Corrêa do Amaral, Rafaely Ferreira Severo, Rafael Sarkis-Onofre, Gustavo Giacomelli Nascimento, Gabriele Cordenonzi, Rachel Krolow Bast, Flávio Fernando Demarco, Fernanda Nedel
Braz. oral res.. 2017-12-18; 31:
DOI: 10.1590/1807-3107bor-2017.vol31.0087

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1. Braz Oral Res. 2017 Dec 18;31:e87. doi: 10.1590/1807-3107BOR-2017.vol31.0087.

How has dental pulp stem cells isolation been conducted? A scoping review.

Ferrúa CP(1), Centeno EGZ(2), Rosa LCD(1), Amaral CCD(1), Severo RF(1),
Sarkis-Onofre R(3), Nascimento GG(4), Cordenonzi G(1), Bast RK(5), Demarco
FF(4), Nedel F(1).

Author information:
(1)Universidade Católica de Pelotas – UCPel, Program in Health and Behavior,
Pelotas, RS, Brazil.
(2)Universidade Federal de Pelotas – UFPel, Technology Development Center,
Graduate Program in Biotecnology, Pelotas, RS, Brazil.
(3)IMED, Graduate Program in Dentistry, Passo Fundo, RS, Brazil.
(4)Universidade Federal de Pelotas – UFPel, Graduate Program in Dentistry,
Pelotas, RS, Brazil.
(5)Universidade Federal do Rio Grande do Sul – UFRGS, Institute of Basic Health
Scieces, Biochemistry Department, Porto Alegre, RS, Brazil.

The objective of this study was to realize a scoping review the literature in
order to identify the profile of DPSCs isolation and analyze the possible risk
factors that could change the native behavior of these cells. An initial search
was conducted using the following MeSH terms: « (dental pulp stem cell [MeSH]) »;
« (dental pulp [MeSH]) » AND « (stem cell [MeSH]) »; « (« dental pulp stem cell »
[MeSH] ») ». The electronic search was done without date restriction up to and
including April 2014, in PubMed, Scopus, Scielo and ISI Web of Knowledge
databases. Studies were submitted to inclusion and exclusion criteria and 222
articles were included. Data showed that over the past 15 years many studies
have been conducted using DPSCs. However this is the first systematic review
regarding the isolation of stem cell, and more specifically of dental pulp stem
cells. The isolation of dental pulp stem cells showed great variability,
hampering the development of standard protocols to achieve in vitro dental pulp
stem cells with similar characteristics. This scoping review combined, for the
first time, the methodologies used for dental pulp stem isolation, highlighting
the most frequently used.

DOI: 10.1590/1807-3107BOR-2017.vol31.0087
PMID: 29267658 [Indexed for MEDLINE]

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