Glutamatergic and GABAergic effects of fipronil on olfactory learning and memory in the honeybee

Abdessalam Kacimi El Hassani, Julien Pierre Dupuis, Monique Gauthier, Catherine Armengaud
Invert Neurosci. 2009-10-23; 9(2): 91-100
DOI: 10.1007/s10158-009-0092-z

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1. Invert Neurosci. 2009 Nov;9(2):91-100. doi: 10.1007/s10158-009-0092-z. Epub 2009
Oct 23.

Glutamatergic and GABAergic effects of fipronil on olfactory learning and memory
in the honeybee.

El Hassani AK(1), Dupuis JP, Gauthier M, Armengaud C.

Author information:
(1)Centre de Recherches sur Cognition Animale, Université Paul Sabatier Toulouse
III-CNRS UMR 5169, 118 Route de Narbonne, 31062 Toulouse Cedex 04, France.

We investigated here the role of transmissions mediated by GABA and
glutamate-gated chloride channels (GluCls) in olfactory learning and memory in
honeybees, both of these channels being a target for fipronil. To do so, we
combined olfactory conditioning with injections of either the GABA- and
glutamate-interfering fipronil alone, or in combination with the blocker of
glutamate transporter L-trans-Pyrrolidine-2,4-Dicarboxylicacid (L-trans-PDC), or
the GABA analog Trans-4-Aminocrotonic Acid (TACA). Our results show that a low
dose of fipronil (0.1 ng/bee) impaired olfactory memory, while a higher dose (0.5
ng/bee) had no effect. The detrimental effect induced by the low dose of fipronil
was rescued by the coinjection of L-trans-PDC but was rather increased by the
coinjection of TACA. Moreover, using whole-cell patch-clamp recordings, we
observed that L-trans-PDC reduced glutamate-induced chloride currents in antennal
lobe cells. We interpret these results as reflecting the involvement of both
GluCl and GABA receptors in the impairment of olfactory memory induced by

DOI: 10.1007/s10158-009-0092-z
PMID: 19851797 [Indexed for MEDLINE]

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