Functional magnetic resonance microscopy at single-cell resolution in Aplysia californica.

G. Radecki, R. Nargeot, I. O. Jelescu, D. Le Bihan, L. Ciobanu
Proceedings of the National Academy of Sciences. 2014-05-28; 111(23): 8667-8672
DOI: 10.1073/pnas.1403739111

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1. Proc Natl Acad Sci U S A. 2014 Jun 10;111(23):8667-72. doi:
10.1073/pnas.1403739111. Epub 2014 May 28.

Functional magnetic resonance microscopy at single-cell resolution in Aplysia
californica.

Radecki G(1), Nargeot R(2), Jelescu IO(1), Le Bihan D(1), Ciobanu L(3).

Author information:
(1)NeuroSpin, Commissariat à l’Energie Atomique et aux Energies Alternatives,
91191 Gif-sur-Yvette, France; and.
(2)Université de Bordeaux andCentre National de la Recherche Scientifique,
Institut de Neurosciences Cognitives et Intégratives d’Aquitaine, Unité Mixte de
Recherche 5287, F-33000 Bordeaux, France.
(3)NeuroSpin, Commissariat à l’Energie Atomique et aux Energies Alternatives,
91191 Gif-sur-Yvette, France; and .

In this work, we show the feasibility of performing functional MRI studies with
single-cell resolution. At ultrahigh magnetic field, manganese-enhanced magnetic
resonance microscopy allows the identification of most motor neurons in the
buccal network of Aplysia at low, nontoxic Mn(2+) concentrations. We establish
that Mn(2+) accumulates intracellularly on injection into the living Aplysia and
that its concentration increases when the animals are presented with a sensory
stimulus. We also show that we can distinguish between neuronal activities
elicited by different types of stimuli. This method opens up a new avenue into
probing the functional organization and plasticity of neuronal networks involved
in goal-directed behaviors with single-cell resolution.

DOI: 10.1073/pnas.1403739111
PMCID: PMC4060695
PMID: 24872449 [Indexed for MEDLINE]

Auteurs Bordeaux Neurocampus