Femoral epiphyseal cartilage matrix changes at predilection sites of equine osteochondrosis: Quantitative MRI, second-harmonic microscopy, and histological findings
J. Orthop. Res.. 2016-02-08; 34(10): 1743-1752
DOI: 10.1002/jor.23176
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Martel G(1), Couture CA(2), Gilbert G(3), Bancelin S(2), Richard H(1), Moser T(4), Kiss S(4), Légaré F(2), Laverty S(5).
Author information:
(1)Comparative Orthopaedic Research Laboratory, Faculté de médecine vétérinaire, Université de Montréal, Québec, Canada.
(2)INRS-EMT, Université du Québec, Québec, Canada.
(3)MR Clinical Science, Philips Healthcare, Markham, Ontario, Canada.
(4)Department of Radiology, Centre Hospitalier de l’Université de Montréal, Hôpital Notre-Dame, Montréal, Québec, Canada.
(5)Comparative Orthopaedic Research Laboratory, Faculté de médecine vétérinaire, Université de Montréal, Québec, Canada.
Osteochondrosis is an ischemic chondronecrosis of epiphyseal growth cartilage
that results in focal failure of endochondral ossification and osteochondritis
dissecans at specific sites in the epiphyses of humans and animals, including
horses. The upstream events leading to the focal ischemia remain unknown. The
epiphyseal growth cartilage matrix is composed of proteoglycan and collagen
macromolecules and encases its vascular tree in canals. The matrix undergoes
major dynamic changes in early life that could weaken it biomechanically and
predispose it to focal trauma and vascular failure. Subregions in neonatal foal
femoral epiphyses (n = 10 osteochondrosis predisposed; n = 6 control) were
assessed for proteoglycan and collagen structure/content employing 3T
quantitative MRI (3T qMRI: T1ρ and T2 maps). Site-matched validations were made
with histology, immunohistochemistry, and second-harmonic microscopy. Growth
cartilage T1ρ and T2 relaxation times were significantly increased (p