Fast and reversible trapping of surface glycine receptors by gephyrin

J. Meier, C. Vannier, A. Sergé, A. Triller, D. Choquet
Nat Neurosci. 2001-03-01; 4(3): 253-260
DOI: 10.1038/85099

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1. Nat Neurosci. 2001 Mar;4(3):253-60.

Fast and reversible trapping of surface glycine receptors by gephyrin.

Meier J(1), Vannier C, Sergé A, Triller A, Choquet D.

Author information:
(1)Biologie Cellulaire de la Synapse N&P INSERM U497, Ecole Normale Supérieure
46, rue d’Ulm 75005, Paris, France.

Comment in
Nat Neurosci. 2001 Mar;4(3):219-20.

Variations in receptor number at a given synapse are known to contribute to
synaptic plasticity, but methods used to establish this idea usually do not allow
for the determination of the dynamics of these phenomena. We used single-particle
tracking to follow in real time, on the cell surface, movements of the glycine
receptor (GlyR) with or without the GlyR stabilizing protein gephyrin. GlyR
alternated within seconds between diffusive and confined states. In the absence
of gephyrin, GlyR were mostly freely diffusing. Gephyrin induced long confinement
periods spatially associated with submembranous clusters of gephyrin. However,
even when most receptors were stabilized, they still frequently made transitions
through the diffusive state. These data show that receptor number in a cluster
results from a dynamic equilibrium between the pools of stabilized and freely
mobile receptors. Modification of this equilibrium could be involved in
regulation of the number of receptors at synapses.

DOI: 10.1038/85099
PMID: 11224541 [Indexed for MEDLINE]

Auteurs Bordeaux Neurocampus