Fast and Easy Enzyme Immobilization by Photoinitiated Polymerization for Efficient Bioelectrochemical Devices
Anal. Chem.. 2011-04-01; 83(7): 2824-2828
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1. Anal Chem. 2011 Apr 1;83(7):2824-8. doi: 10.1021/ac200297r. Epub 2011 Mar 15.
Fast and easy enzyme immobilization by photoinitiated polymerization for
efficient bioelectrochemical devices.
Suraniti E(1), Studer V, Sojic N, Mano N.
(1)Université de Bordeaux, Centre de Recherche Paul Pascal, CNRS UPR 8641, 115
avenue Schweitzer, 33600 Pessac, France.
Immobilization and electrical wiring of enzymes is of particular importance for
the elaboration of efficient biosensors and can be cumbersome. Here, we report a
fast and easy protocol for enzyme immobilization, and as a proof of concept, we
applied it to the immobilization of bilirubin oxidase, a labile enzyme. In the
first step, bilirubin oxidase is mixed with a redox hydrogel “wiring” the enzyme
reaction centers to electrodes. Then, this adduct is covered by an outer layer of
PEGDA made by photoinitiated polymerization of poly(ethylene-glycol) diacrylate
(PEGDA) and a photoclivable precursor, DAROCUR. This two-step protocol is 18
times faster than the current state-of-the-art protocol and leads to currents 25%
higher. In addition, the outer layer of PEGDA acts as a protective layer
increasing the lifetime of the electrode by 100% when operating continuously for
2000 s and by 60% when kept in dry state for 24 h. This new protocol is
particularly appropriate for labile enzymes that quickly denaturate. In addition,
by tuning the ratio PEGDA/DAROCUR, it is possible to make the enzyme electrodes
even more active or more stable.
PMID: 21405108 [Indexed for MEDLINE]