F3 neuronal adhesion molecule controls outgrowth and fasciculation of cerebellar granule cell neurites: a cell-type-specific effect mediated by the Ig-like domains.
Molecular and Cellular Neuroscience. 1996-07-01; 8(1): 53-69
DOI: 10.1006/mcne.1996.0043
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1. Mol Cell Neurosci. 1996;8(1):53-69. doi: 10.1006/mcne.1996.0043.
F3 neuronal adhesion molecule controls outgrowth and fasciculation of cerebellar
granule cell neurites: a cell-type-specific effect mediated by the Ig-like
domains.
Buttiglione M(1), Revest JM, Rougon G, Faivre-Sarrailh C.
Author information:
(1)Laboratoire de Génétique et de Physiologie du Développement, UMR 9943 CNRS,
Parc Scientifique de Luminy, Marseille, France.
F3 is a glycane phosphatidylinositol-anchored neuronal adhesion glycoprotein
which consists of immunoglobulin (Ig) domains and fibronectin type III repeats.
Here we showed that total F3 or F3-Ig domains when presented as membrane
components of CHO transfected cells influenced growth cone morphology, strongly
inhibited outgrowth, and induced fasciculation of cerebellar granule cell axons.
An F3-Ig-Fc chimera induced neurite fasciculation from cerebellar neuron
aggregates when used as a coated substrate but not in the soluble form. The F3
effect on neurite elongation is highly specific for neuronal cell types since
under the same experimental conditions it did not modify neurite outgrowth of
hippocampal neurons and was shown to stimulate elongation of neurites from
sensory neurons in both membrane-anchored and soluble form. Our results provide
evidence to extend the proposed role of F3 and strongly suggest that
axonal-growth-controlling molecules may quite generally exert dual actions which
are likely to depend on the receptor repertoire of the responding neuron.