Expression of the glycinergic system during the course of embryonic development in the mouse spinal cord and its co-localization with GABA immunoreactivity
J. Comp. Neurol.. 2006-01-01; 496(6): 832-846
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1. J Comp Neurol. 2006 Jun 20;496(6):832-46.
Expression of the glycinergic system during the course of embryonic development
in the mouse spinal cord and its co-localization with GABA immunoreactivity.
Allain AE(1), Baïri A, Meyrand P, Branchereau P.
(1)Laboratoire de Neurobiologie des Réseaux, Université Bordeaux 1 et Centre
National de la Recherche Scientifique, Unité Mixte de Recherche 5816, Talence,
To understand better the role of glycine and gamma-aminobutyric acid (GABA) in
the mouse spinal cord during development, we previously described the ontogeny of
GABA. Now, we present the ontogeny of glycine-immunoreactive (Gly-ir) somata and
fibers, at brachial and lumbar levels, from embryonic day 11.5 (E11.5) to
postnatal day 0 (P0). Spinal Gly-ir somata appeared at E12.5 in the ventral horn,
with a higher density at the brachial level. They were intermingled with numerous
Gly-ir fibers reaching the border of the marginal zone. By E13.5, at the brachial
level, the number of Gly-ir perikarya sharply increased throughout the whole
ventral horn, whereas the density of fibers declined in the marginal zone. In the
dorsal horn, the first Gly-ir somata were then detected. From E13.5 to E16.5, at
the brachial level, the density of Gly-ir cells remained stable in the ventral
horn, and after E16.5 it decreased to reach a plateau. In the dorsal horn, the
density of Gly-ir cells increased, and after E16.5 it remained stable. At the
lumbar level, maximum expression was reached at E16.5 in both the ventral and
dorsal horn. Finally, the co-localization of glycine and GABA was analyzed, in
the ventral motor area, at E13.5, E15.5, and E17.5. The results showed that,
regardless of developmental stage studied, one-third of the stained somata
co-expressed GABA and glycine. Our data show that the glycinergic system matures
1 day later than the GABAergic system and follows a parallel spatiotemporal
evolution, leading to a larger population of glycine cells in the ventral horn.
Copyright 2006 Wiley-Liss, Inc.
PMID: 16628621 [Indexed for MEDLINE]