Dynamic phase transitions in cell spreading.
Phys. Rev. Lett.. 2004-09-02; 93(10):
DOI: 10.1103/physrevlett.93.108105
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1. Phys Rev Lett. 2004 Sep 3;93(10):108105. Epub 2004 Sep 2.
Dynamic phase transitions in cell spreading.
Döbereiner HG(1), Dubin-Thaler B, Giannone G, Xenias HS, Sheetz MP.
Author information:
(1)Department of Biological Sciences, Columbia University, New York, New York
10027, USA.
We monitored isotropic spreading of mouse embryonic fibroblasts on
fibronectin-coated substrates. Cell adhesion area versus time was measured via
total internal reflection fluorescence microscopy. Spreading proceeds in
well-defined phases. We found a power-law area growth with distinct exponents in
three sequential phases, which we denote as basal, continuous, and contractile
spreading. High resolution differential interference contrast microscopy was used
to characterize local membrane dynamics at the spreading front. Fourier power
spectra of membrane velocity reveal the sudden development of periodic membrane
retractions at the transition from continuous to contractile spreading. We
propose that the classification of cell spreading into phases with distinct
functional characteristics and protein activity serves as a paradigm for a
general program of a phase classification of cellular phenotype.
DOI: 10.1103/PhysRevLett.93.108105
PMID: 15447457 [Indexed for MEDLINE]