Directed actin assembly and motility.

Rajaa Boujemaa-Paterski, Rémi Galland, Cristian Suarez, Christophe Guérin, Manuel Théry, Laurent Blanchoin
Methods in Enzymology. 2014-01-01; : 283-300
DOI: 10.1016/b978-0-12-397924-7.00016-9

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1. Methods Enzymol. 2014;540:283-300. doi: 10.1016/B978-0-12-397924-7.00016-9.

Directed actin assembly and motility.

Boujemaa-Paterski R(1), Galland R(2), Suarez C(2), Guérin C(2), Théry M(2),
Blanchoin L(2).

Author information:
(1)Institut de Recherches en Technologies et Sciences pour le Vivant, iRTSV,
Laboratoire de Physiologie Cellulaire et Végétale, CNRS/CEA/INRA/UJF, Grenoble,
France. Electronic address: *protected email*.
(2)Institut de Recherches en Technologies et Sciences pour le Vivant, iRTSV,
Laboratoire de Physiologie Cellulaire et Végétale, CNRS/CEA/INRA/UJF, Grenoble,
France.

The actin cytoskeleton is a key component of the cellular architecture. However,
understanding actin organization and dynamics in vivo is a complex challenge.
Reconstitution of actin structures in vitro, in simplified media, allows one to
pinpoint the cellular biochemical components and their molecular interactions
underlying the architecture and dynamics of the actin network. Previously, little
was known about the extent to which geometrical constraints influence the dynamic
ultrastructure of these networks. Therefore, in order to study the balance
between biochemical and geometrical control of complex actin organization, we
used the innovative methodologies of UV and laser patterning to design a wide
repertoire of nucleation geometries from which we assembled branched actin
networks. Using these methods, we were able to reconstitute complex actin network
organizations, closely related to cellular architecture, to precisely direct and
control their 3D connections. This methodology mimics the actin networks
encountered in cells and can serve in the fabrication of innovative bioinspired
systems.

© 2014 Elsevier Inc. All rights reserved.

DOI: 10.1016/B978-0-12-397924-7.00016-9
PMID: 24630113 [Indexed for MEDLINE]


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