Differential effect of maternal diet supplementation with α-linolenic acid or n-3 long-chain polyunsaturated fatty acids on glial cell phosphatidylethanolamine and phosphatidylserine fatty acid profile in neonate rat brains
Nutrition & Metabolism. 2010-01-01; 7(1): 2
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1. Nutr Metab (Lond). 2010 Jan 14;7:2. doi: 10.1186/1743-7075-7-2.
Differential effect of maternal diet supplementation with alpha-Linolenic adcid
or n-3 long-chain polyunsaturated fatty acids on glial cell
phosphatidylethanolamine and phosphatidylserine fatty acid profile in neonate rat
Destaillats F(1), Joffre C, Acar N, Joffre F, Bezelgues JB, Pasquis B,
Cruz-Hernandez C, Rezzi S, Montoliu I, Dionisi F, Bretillon L.
(1)Nestlé Research Center, Vers-chez-les-Blanc, Lausanne, Switzerland.
BACKGROUND: Dietary long-chain polyunsaturated fatty acids (LC-PUFA) are of
crucial importance for the development of neural tissues. The aim of this study
was to evaluate the impact of a dietary supplementation in n-3 fatty acids in
female rats during gestation and lactation on fatty acid pattern in brain glial
cells phosphatidylethanolamine (PE) and phosphatidylserine (PS) in the neonates.
METHODS: Sprague-Dawley rats were fed during the whole gestation and lactation
period with a diet containing either docosahexaenoic acid (DHA, 0.55%) and
eicosapentaenoic acid (EPA, 0.75% of total fatty acids) or alpha-linolenic acid
(ALA, 2.90%). At two weeks of age, gastric content and brain glial cell PE and PS
of rat neonates were analyzed for their fatty acid and dimethylacetal (DMA)
profile. Data were analyzed by bivariate and multivariate statistics.
RESULTS: In the neonates from the group fed with n-3 LC-PUFA, the DHA level in
gastric content (+65%, P < 0.0001) and brain glial cell PE (+18%, P = 0.0001) and
PS (+15%, P = 0.0009) were significantly increased compared to the ALA group. The
filtered correlation analysis (P < 0.05) underlined that levels of
dihomo-gamma-linolenic acid (DGLA), DHA and n-3 docosapentaenoic acid (DPA) were
negatively correlated with arachidonic acid (ARA) and n-6 DPA in PE of brain
glial cells. No significant correlation between n-3 and n-6 LC-PUFA were found in
the PS dataset. DMA level in PE was negatively correlated with n-6 DPA. DMA were
found to occur in brain glial cell PS fraction; in this class DMA level was
correlated negatively with DHA and positively with ARA.
CONCLUSION: The present study confirms that early supplementation of maternal
diet with n-3 fatty acids supplied as LC-PUFA is more efficient in increasing n-3
in brain glial cell PE and PS in the neonate than ALA. Negative correlation
between n-6 DPA, a conventional marker of DHA deficiency, and DMA in PE suggests
n-6 DPA that potentially be considered as a marker of tissue ethanolamine
plasmalogen status. The combination of multivariate and bivariate statistics
allowed to underline that the accretion pattern of n-3 LC-PUFA in PE and PS