Dietary n-3 polyunsaturated fatty acid deficiency alters olfactory mucosa sensitivity in young mice but has no impact on olfactory behavior
Nutritional Neuroscience. 2022-06-11; 26(8): 706-719
DOI: 10.1080/1028415x.2022.2082642
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1. Nutr Neurosci. 2023 Aug;26(8):706-719. doi: 10.1080/1028415X.2022.2082642.
Epub 2022 Jun 11.
Dietary n-3 polyunsaturated fatty acid deficiency alters olfactory mucosa
sensitivity in young mice but has no impact on olfactory behavior.
Soubeyre V(1), Merle L(2), Jarriault D(3), Grégoire S(2), Bretillon L(2), Acar
N(2), Grosmaitre X(2), Le Bon AM(2).
Author information:
(1)Institut de Génomique Fonctionnelle, Université de Montpellier, CNRS
UMR-5203, INSERM U1091, Montpellier, France.
(2)Centre des Sciences du Goût et de l’Alimentation, AgroSup Dijon, CNRS, INRAE,
Université Bourgogne Franche-Comté, Dijon, France.
(3)NutriNeuro, UMR 1286 INRAE, Bordeaux INP, Université de Bordeaux, Bordeaux,
France.
BACKGROUND AND OBJECTIVE: We recently showed that perinatal exposure to diets
with unbalanced n-6:n-3 polyunsaturated fatty acid (PUFA) ratios affects the
olfactory mucosa (OM) fatty acid composition. To assess the repercussions of
these modifications, we investigated the impact of diets unbalanced in n-3 PUFAs
on the molecular composition and functionality of the OM in young mice.
METHODS: After mating, female mice were fed diets either deficient in
α-linolenic acid (LOW diet) or supplemented with n-3 long-chain PUFAs (HIGH
diet) during the perinatal period. Weaned male offspring were then fed ad
libitum with the same experimental diets for 5 weeks. At 8 weeks of age,
olfactory behavior tests were performed in young mice. The fatty acid
composition of OM and olfactory cilia, as well as the expression of genes
involved in different cellular pathways, were analyzed. The electroolfactograms
induced by odorant stimuli were recorded to assess the impact of diets on OM
functionality.
RESULTS AND CONCLUSION: Both diets significantly modified the fatty acid
profiles of OM and olfactory cilia in young mice. They also induced changes in
the expression of genes involved in olfactory signaling and in olfactory neuron
maturation. The electroolfactogram amplitudes were reduced in mice fed the LOW
diet. Nevertheless, the LOW diet and the HIGH diet did not affect mouse
olfactory behavior. Our study demonstrated that consumption of diets deficient
in or supplemented with n-3 PUFAs during the perinatal and postweaning periods
caused significant changes in young mouse OM. However, these modifications did
not impair their olfactory capacities.
DOI: 10.1080/1028415X.2022.2082642
PMID: 35694841 [Indexed for MEDLINE]