Determination of collagen fiber orientation in histological slides using Mueller microscopy and validation by second harmonic generation imaging
Opt. Express. 2014-09-10; 22(19): 22561
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1. Opt Express. 2014 Sep 22;22(19):22561-74. doi: 10.1364/OE.22.022561.
Determination of collagen fiber orientation in histological slides using Mueller
microscopy and validation by second harmonic generation imaging.
Bancelin S, Nazac A, Ibrahim BH, Dokládal P, Decencière E, Teig B, Haddad H,
Fernandez H, Schanne-Klein MC, De Martino A.
We studied the azimuthal orientations of collagen fibers in histological slides
of uterine cervical tissue by two different microscopy techniques, namely Mueller
polarimetry (MP) and Second Harmonic Generation (SHG). SHG provides direct
visualization of the fibers with high specificity, which orientations is then
obtained by suitable image processing. MP provides images of retardation (among
other polarimetric parameters) due to the optical anisotropy of the fibers, which
is enhanced by Picrosirius Red staining. The fiber orientations are then assumed
to be those of the retardation slow axes. The two methods, though fully different
from each other, provide quite similar maps of average fiber orientations.
Overall, our results confirm that MP microscopy provides reliable images of
dominant fiber orientations at a much lower cost that SHG, which remains the
« gold standard » for specific imaging of collagen fibers using optical microscopy.
PMID: 25321725 [Indexed for MEDLINE]