Determination of cerebrospinal fluid production rate using a push‐pull perfusion procedure in the conscious rat

V. Baudrie, JB Roullet, Y. Goureau, F. Chaouloff, JL Elghozi
Fundamental & Clinical Pharmacology. 1990-05-06; 4(3): 269-274
DOI: 10.1111/j.1472-8206.1990.tb00494.x


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Baudrie V(1), Roullet JB, Goureau Y, Chaouloff F, Elghozi JL.

Author information:
(1)Laboratoire de Pharmacologie, CNRS I 61670, Faculté de Médecine Necker-Enfants
Malades, Paris, France.

The inulin dilution technique was used to determine the cerebrospinal fluid (CSF)
production rate by means of a push-pull cannula implanted into a lateral
ventricle. Artificial CSF containing trace amount of 3H-inulin was perfused for 2
h in conscious rats. 3H-inulin in the effluent reached a plateau level depending
on the CSF production rate. The control lateroventricular CSF production was 0.98
microliters/min. Production was reduced to 0.34 microliters/min during a
perfusion with acetazolamide (1 mM), a carbonic anhydrase inhibitor.

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