Critical study of common conditions of storage of glucocorticoids and catecholamines in 24-h urine collected during resting and exercising conditions.
Clinica Chimica Acta. 2004-10-01; 348(1-2): 207-214
DOI: 10.1016/j.cccn.2004.05.023
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1. Clin Chim Acta. 2004 Oct;348(1-2):207-14.
Critical study of common conditions of storage of glucocorticoids and
catecholamines in 24-h urine collected during resting and exercising conditions.
Gouarne C(1), Foury A, Duclos M.
Author information:
(1)Laboratory of Neurogenetic and Stress, INSERM-INRA-University of Bordeaux II,
Institute François Magendie, Camille St Saëns Street, 33077 Bordeaux Cedex,
France.
BACKGROUND: Except immediate freezing of the samples, no practical method has
been validated for preservation of glucocorticoids and catecholamines in 24-h
urine collection. Furthermore, the influence of urine storage at bladder
temperature during periods of different lengths and the effect of prior exercise
on preservation of these hormones in the bladder have not been investigated until
now.
METHODS: Ten healthy volunteers collected their urine both after a resting and
after an exercise session. Urine was aliquoted into tubes which were stored
during 24 h in the presence or in the absence of preservatives and at different
temperatures. Two samples were stored either 3 or 9 h at 37 degrees C (bladder
temperature) without additive.
RESULTS AND CONCLUSIONS: When collecting 24-h urine samples for glucocorticoids
determination, sample can be stored at room temperature during the 24-h
collection period without compromising glucocorticoids preservation. When
collecting 24-h urine samples for catecholamines determination, samples have to
be chilled without preservative during the whole of the collection period. If the
samples have to be stored at room temperature, HCl should be used. Moreover, we
report for the first time that catecholamines can be degraded in the bladder and
therefore that subjects should urinate every 3 h during either a resting or an
exercising day.
DOI: 10.1016/j.cccn.2004.05.023
PMID: 15369756 [Indexed for MEDLINE]