Coupling ion channels to receptors for biomolecule sensing
Nature Nanotech. 2008-09-07; 3(10): 620-625
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1. Nat Nanotechnol. 2008 Oct;3(10):620-5. doi: 10.1038/nnano.2008.242. Epub 2008 Sep
Coupling ion channels to receptors for biomolecule sensing.
Moreau CJ(1), Dupuis JP, Revilloud J, Arumugam K, Vivaudou M.
(1)Laboratoire des Protéines Membranaires, Institut de Biologie Structurale (CEA,
CNRS, UJF), 41 rue Jules Horowitz, 38027 Grenoble, France.
Nat Nanotechnol. 2008 Oct;3(10):587-8.
Nanoscale electrical biosensors are promising tools for diagnostics and
high-throughput screening systems. The electrical signal allows label-free assays
with a high signal-to-noise ratio and fast real-time measurements. The challenge
in developing such biosensors lies in functionally connecting a molecule detector
to an electrical switch. Advances in this field have relied on synthetic
ion-conducting pores and modified ion channels that are not yet suitable for
biomolecule screening. Here we report the design and characterization of a novel
bioelectric-sensing platform engineered by coupling an ion channel, which serves
as the electrical probe, to G-protein-coupled receptors (GPCRs), a family of
receptors that detect molecules outside the cell. These ion-channel-coupled
receptors may potentially detect a wide range of ligands recognized by natural or
altered GPCRs, which are known to be major pharmaceutical targets. This could
form a unique platform for label-free drug screening.
PMID: 18839002 [Indexed for MEDLINE]