Contactin-associated protein 1 (Caspr1) regulates the traffic and synaptic content of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptors

Sandra D. Santos, Olga Iuliano, Luís Ribeiro, Julien Veran, Joana S. Ferreira, Pedro Rio, Christophe Mulle, Carlos B. Duarte, Ana Luísa Carvalho
J. Biol. Chem.. 2012-01-05; 287(9): 6868-6877
DOI: 10.1074/jbc.M111.322909

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1. J Biol Chem. 2012 Feb 24;287(9):6868-77. doi: 10.1074/jbc.M111.322909. Epub 2012
Jan 5.

Contactin-associated protein 1 (Caspr1) regulates the traffic and synaptic
content of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type
glutamate receptors.

Santos SD(1), Iuliano O, Ribeiro L, Veran J, Ferreira JS, Rio P, Mulle C, Duarte
CB, Carvalho AL.

Author information:
(1)CNC-Center for Neuroscience and Cell Biology, University of Coimbra, 3004-517
Coimbra, Portugal.

Glutamate receptors of the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid
(AMPA) type mediate fast excitatory synaptic transmission in the CNS. Synaptic
strength is modulated by AMPA receptor binding partners, which regulate receptor
synaptic targeting and functional properties. We identify Contactin-associated
protein 1 (Caspr1) as an AMPA receptor interactor. Caspr1 is present in synapses
and interacts with AMPA receptors in brain synaptic fractions. Coexpression of
Caspr1 with GluA1 increases the amplitude of glutamate-evoked currents. Caspr1
overexpression in hippocampal neurons increases the number and size of synaptic
GluA1 clusters, whereas knockdown of Caspr1 decreases the intensity of synaptic
GluA1 clusters. Hence, Caspr1 is a regulator of the trafficking of AMPA receptors
to synapses.

DOI: 10.1074/jbc.M111.322909
PMCID: PMC3307290
PMID: 22223644 [Indexed for MEDLINE]


Auteurs Bordeaux Neurocampus