Concentration landscape generators for shear free dynamic chemical stimulation
Lab Chip. 2012-01-01; 12(7): 1340
DOI: 10.1039/c2lc20994b
Lire sur PubMed
1. Lab Chip. 2012 Apr 7;12(7):1340-6. doi: 10.1039/c2lc20994b. Epub 2012 Feb 20.
Concentration landscape generators for shear free dynamic chemical stimulation.
Morel M(1), Galas JC, Dahan M, Studer V.
Author information:
(1)Laboratoire Kastler Brossel, CNRS UMR8552, Département de Physique et Institut
de Biologie, Ecole Normale Supérieure, Université Pierre et Marie Curie – Paris
6, 46 rue d’Ulm, 75005 Paris, France.
In this paper we first introduce a novel fabrication process, which allows for
easy integration of thin track-etched nanoporous membranes, within 2D or 3D
microchannel networks. In these networks, soluble chemical compounds can diffuse
out of the channels through well-defined and spatially organized microfabricated
porous openings. Interestingly, multiple micron-scale porous areas can be
integrated in the same device and each of these areas can be connected to a
different microfluidic channel and reservoir. We then present and characterize
several membrane-based microdevices and their use for the generation of stable
diffusible concentration gradients and complex dynamic chemical landscapes under
shear free conditions. We also demonstrate how a simple flow-focusing geometry
can be used to generate « on-demand » concentration profiles. In turn, these
devices should provide an ideal experimental framework for high throughput
cell-based assays: long term high-resolution video microscopy experiments can be
performed, under multiple spatially and temporally controlled chemical
conditions, with simple protocols and in a cell-friendly environment.
This journal is © The Royal Society of Chemistry 2012
DOI: 10.1039/c2lc20994b
PMID: 22344388 [Indexed for MEDLINE]