Co-culture of Glioblastoma Stem-like Cells on Patterned Neurons to Study Migration and Cellular Interactions
JoVE. 2021-02-24; (168):
DOI: 10.3791/62213
Lire sur PubMed
https://www.bordeaux-neurocampus.fr/12069
Guyon J(1), Strale PO(2), Romero-Garmendia I(3), Bikfalvi A(1), Studer V(#)(4),
Daubon T(#)(5).
Author information:
(1)University Bordeaux, INSERM, LAMC, U1029, 33600, Pessac, France.
(2)Univ. Bordeaux, CNRS, Interdisciplinary Institute for Neuroscience, IINS, UMR
5297, Bordeaux, France; Alvéole, Bordeaux, France.
(3)University Bordeaux, CNRS, IBGC, UMR5095, 33000, Bordeaux, France.
(4)Univ. Bordeaux, CNRS, Interdisciplinary Institute for Neuroscience, IINS, UMR
5297, Bordeaux, France.
(5)University Bordeaux, CNRS, IBGC, UMR5095, 33000, Bordeaux, France;
.
(#)Contributed equally
Glioblastomas (GBMs), grade IV malignant gliomas, are one of the deadliest types
of human cancer because of their aggressive characteristics. Despite significant
advances in the genetics of these tumors, how GBM cells invade the healthy brain
parenchyma is not well understood. Notably, it has been shown that GBM cells
invade the peritumoral space via different routes; the main interest of this
paper is the route along white matter tracts (WMTs). The interactions of tumor
cells with the peritumoral nervous cell components are not well characterized.
Herein, a method has been described that evaluates the impact of neurons on GBM
cell invasion. This paper presents an advanced co-culture in vitro assay that
mimics WMT invasion by analyzing the migration of GBM stem-like cells on
neurons. The behavior of GBM cells in the presence of neurons is monitored by
using an automated tracking procedure with open-source and free-access software.
This method is useful for many applications, in particular, for functional and
mechanistic studies as well as for analyzing the effects of pharmacological
agents that can block GBM cell migration on neurons.
DOI: 10.3791/62213
PMID: 33720119 [Indexed for MEDLINE]