Characterization of cutaneous and articular sensory neurons

Ines da Silva Serra, Zoé Husson, Jonathan D. Bartlett, Ewan St. John Smith
Mol Pain. 2016-01-01; 12: 174480691663638
DOI: 10.1177/1744806916636387

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1. Mol Pain. 2016 Mar 15;12. pii: 1744806916636387. doi: 10.1177/1744806916636387.

da Silva Serra I(1), Husson Z(2), Bartlett JD(2), Smith ES(3).

Author information:
(1)Department of Pharmacology, University of Cambridge, Cambridge, UK School of Psychology and Clinical Language Sciences, University of Reading, Reading, UK.
(2)Department of Pharmacology, University of Cambridge, Cambridge, UK.
(3)Department of Pharmacology, University of Cambridge, Cambridge, UK .

BACKGROUND: A wide range of stimuli can activate sensory neurons and neurons innervating specific tissues often have distinct properties. Here, we used retrograde tracing to identify sensory neurons innervating the hind paw skin (cutaneous) and ankle/knee joints (articular), and combined immunohistochemistry
and electrophysiology analysis to determine the neurochemical phenotype of cutaneous and articular neurons, as well as their electrical and chemical excitability.

RESULTS: Immunohistochemistry analysis using RetroBeads as a retrograde tracer confirmed previous data that cutaneous and articular neurons are a mixture of myelinated and unmyelinated neurons, and the majority of both populations are peptidergic. In whole-cell patch-clamp recordings from cultured dorsal root
ganglion neurons, voltage-gated inward currents and action potential parameters were largely similar between articular and cutaneous neurons, although cutaneous neuron action potentials had a longer half-peak duration (HPD). An assessment of chemical sensitivity showed that all neurons responded to a pH 5.0 solution, but that acid-sensing ion channel (ASIC) currents, determined by inhibition with the nonselective acid-sensing ion channel antagonist benzamil, were of a greater magnitude in cutaneous compared to articular neurons. Forty to fifty percent of cutaneous and articular neurons responded to capsaicin, cinnamaldehyde, and menthol, indicating similar expression levels of transient receptor potential vanilloid 1 (TRPV1), transient receptor potential ankyrin 1 (TRPA1), and transient receptor potential melastatin 8 (TRPM8), respectively. By contrast, significantly more articular neurons responded to ATP than cutaneous neurons.

CONCLUSION: This work makes a detailed characterization of cutaneous and articular sensory neurons and highlights the importance of making recordings from identified neuronal populations: sensory neurons innervating different tissues have subtly different properties, possibly reflecting different functions.

© The Author(s) 2016.

DOI: 10.1177/1744806916636387
PMCID: PMC4956179
PMID: 27030722 [Indexed for MEDLINE]

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