CEREBRAL TRYPTOPHAN-HYDROXYLASE ACTIVITY, AND 5-HT1A RECEPTOR, 5-HT2A, RECEPTOR, AND 5-HT TRANSPORTER BINDING IN GROUPED AND ISOLATED ROMAN RHA AND RLA RATS – RELATIONSHIPS WITH BEHAVIORS IN 2 MODELS OF ANXIETY
Psychopharmacology. 1995-10-01; 121(3): 385-395
DOI: 10.1007/bf02246079
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1. Psychopharmacology (Berl). 1995 Oct;121(3):385-95.
Cerebral tryptophan hydroxylase activity, and 5-HT1A receptor, 5-HT2A receptor,
and 5-HT transporter binding in grouped and isolated Roman RHA and RLA rats:
relationships with behaviours in two models of anxiety.
Kulikov A(1), Castanon N, Mormède P, Chaouloff F.
Author information:
(1)CJF 94-05 INSERM-INRA, Université Bordeaux II, France.
Male Roman low-(RLA) and high-avoidance (RHA) rats differ when tested in the
elevated plus-maze and the black/white box, but not when (isolated and) tested
for their social interaction. Herein, we have analysed the impact of prior
isolation on male Roman rats tested in the first two models of anxiety; moreover,
because central serotonin (5-HT) systems in Roman rats have been scarcely
studied, we have also analysed several anxiety-related indices of central
serotonergic activity in grouped/isolated Roman rats. Group-housed RLA rats
tested in the elevated plus-maze and the black/white box were less anxious than
their RHA counterparts, thereby confirming our previous study. Isolation had
anxiogenic (and hypolocomotor) effects, these being significant in RLA rats only.
Tryptophan hydroxylase activity in midbrain (but not in cortex, hippocampus or
hypothalamus) was lower in group-housed (but not in isolated) RLA rats than in
RHA rats, a difference independent from changes in the regulatory properties of
the enzyme. Neither midbrain and hippocampal
[3H]8-hydroxy-2-(di-n-propylamino)-tetrlin binding at 5-HT1A receptors, nor
midbrain [3H] citalopram binding at the 5-HT transporter was different between
grouped/isolated RHA/RLA rats. Alternatively, a trend toward a lower hypothalamic
[3H]citalopram binding in (group-housed) RLA rats than in RHA rats could be
noted, whereas cortical [3H]ketanserin binding at 5-HT2A receptors was lower in
RLA rats than in RHA rats, a difference prevented by prior isolation. This study
opens the possibility that inter-line differences in 5-HT2A receptors partly (or
totally) underlie the respective behaviours of RHA and RLA rats in the elevated
plus-maze and the black/white box.
DOI: 10.1007/bf02246079
PMID: 8584622 [Indexed for MEDLINE]