Atrazine and PCB 153 and their effects on the proteome of subcellular fractions of human MCF-7 cells
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics. 2012-06-01; 1824(6): 833-841
Lire sur PubMed
1. Biochim Biophys Acta. 2012 Jun;1824(6):833-41. doi: 10.1016/j.bbapap.2012.03.014.
Epub 2012 Apr 16.
Atrazine and PCB 153 and their effects on the proteome of subcellular fractions
of human MCF-7 cells.
Lasserre JP(1), Fack F, Serchi T, Revets D, Planchon S, Renaut J, Hoffmann L,
Gutleb AC, Muller CP, Bohn T.
(1)Department of Environment and Agro-Biotechnologies, Centre de Recherche Public
– Gabriel Lippmann, Belvaux, Luxembourg.
Several man-made organic pollutants including polychlorinated biphenyls (PCBs)
and several pesticides may exhibit endocrine disrupting (ED) properties. These ED
molecules can be comparatively persistent in the environment, and have shown to
perturb hormonal activity and several physiological functions. The objective of
this investigation was to study the impact of PCB 153 and atrazine on human MCF-7
cells, and to search for marker proteins of their exposure. Cells were exposed to
environmentally high but relevant concentrations of atrazine (200ppb), PCB 153
(500ppb), 17-β estradiol (positive control, 10nM) and DMSO (0.1%, negative
control) for t=36h (n=3 replicates/exposure group). Proteins from cell membrane
and cytosol were isolated, and studied by 2D-DiGE. Differentially regulated
proteins were trypsin-digested and identified by MALDI-ToF-ToF and NCBInr
database. A total of 36 differentially regulated proteins (>|1.5| fold change,