Atomic force microscopy combined with human pluripotent stem cell derived cardiomyocytes for biomechanical sensing.
Biosensors and Bioelectronics. 2016-11-01; 85: 751-757
DOI: 10.1016/j.bios.2016.05.073
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Pesl M(1), Pribyl J(2), Acimovic I(3), Vilotic A(3), Jelinkova S(3), Salykin A(1), Lacampagne A(4), Dvorak P(1), Meli AC(5), Skladal P(6), Rotrekl V(1).
Author information:
(1)Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic; ICRC, St. Anne’s University Hospital, Brno, Czech Republic.
(2)CEITEC, Masaryk University, Brno, Czech Republic.
(3)Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
(4)PhyMedExp, INSERM U1046, University of Montpellier, Montpellier, France.
(5)Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic; PhyMedExp, INSERM U1046, University of Montpellier, Montpellier, France.
(6)CEITEC, Masaryk University, Brno, Czech Republic. Electronic address: .
Cardiomyocyte contraction and relaxation are important parameters of cardiac
function altered in many heart pathologies. Biosensing of these parameters
represents an important tool in drug development and disease modeling. Human
embryonic stem cells and especially patient specific induced pluripotent stem
cell-derived cardiomyocytes are well established as cardiac disease model.. Here,
a live stem cell derived embryoid body (EB) based cardiac cell syncytium served
as a biorecognition element coupled to the microcantilever probe from atomic
force microscope thus providing reliable micromechanical cellular biosensor
suitable for whole-day testing. The biosensor was optimized regarding the type of
cantilever, temperature and exchange of media; in combination with standardized
protocol, it allowed testing of compounds and conditions affecting the
biomechanical properties of EB. The studied effectors included calcium , drugs
modulating the catecholaminergic fight-or-flight stress response such as the
beta-adrenergic blocker metoprolol and the beta-adrenergic agonist isoproterenol.
Arrhythmogenic effects were studied using caffeine. Furthermore, with EBs
originating from patient’s stem cells, this biosensor can help to characterize
heart diseases such as dystrophies.
Copyright © 2016 Elsevier B.V. All rights reserved.