Artificial CSF Motion Ensures Rhythmic Activity in the Developing CNS Ex Vivo: A Mechanical Source of Rhythmogenesis?
Journal of Neuroscience. 2011-06-15; 31(24): 8832-8840
DOI: 10.1523/jneurosci.1354-11.2011
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https://www.bordeaux-neurocampus.fr/12194
1. J Neurosci. 2011 Jun 15;31(24):8832-40. doi: 10.1523/JNEUROSCI.1354-11.2011.
Artificial CSF motion ensures rhythmic activity in the developing CNS ex vivo: a
mechanical source of rhythmogenesis?
Yvert B(1), Mazzocco C, Joucla S, Langla A, Meyrand P.
Author information:
(1)Institut des Neurosciences Cognitives et Intégratives d’Aquitaine, CNRS,
Université de Bordeaux, UMR5287, Talence, F-33405 France.
Erratum in
J Neurosci. 2012 Nov 28;32(48):17524.
Spontaneous rhythmic activity is a ubiquitous feature of developing neural
structures that has been shown to be essential for the establishment of
functional CNS connectivity. However, the primordial origin of these rhythms
remains unknown. Here, we describe two types of rhythmic activity in distinct
parts of the developing CNS isolated ex vivo on microelectrode arrays, the
expression of which was found to be strictly dependent upon the movement of the
artificial CSF (aCSF) flowing over the inner wall of the ventricles or over the
outer surface of the CNS. First, whole embryonic mouse hindbrain-spinal cord
preparations (stages E12.5-E15.5) rhythmically expressed waves of activity
originating in the hindbrain and propagating in the spinal cord. Interestingly
enough, the frequency of this rhythm was completely determined by the speed of
the aCSF flow. In particular, at all stages considered, hindbrain activity was
abolished when the perfusion was stopped. Immature rhythmic activity was also
recorded in the isolated newborn (P0-P8) mouse cortex under normal aCSF
perfusion. Again, this rhythm was abolished when the perfusion flow was stopped.
In both structures, this phenomenon was not due to changes in temperature,
oxygen level, or pH of the bath, but to the movement itself of the aCSF. These
observations challenge the so-called « spontaneous » nature of rhythmic activity
in immature neural networks and suggest that the movement of CSF in the
ventricles and around the brain in vivo may mechanically drive rhythmogenesis in
the developing CNS.
DOI: 10.1523/JNEUROSCI.1354-11.2011
PMCID: PMC6622937
PMID: 21677167 [Indexed for MEDLINE]