An anterograde rabies virus vector for high-resolution large-scale reconstruction of 3D neuron morphology
Brain Struct Funct. 2014-04-11; 220(3): 1369-1379
DOI: 10.1007/s00429-014-0730-z
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1. Brain Struct Funct. 2015;220(3):1369-79. doi: 10.1007/s00429-014-0730-z. Epub
2014 Apr 11.
An anterograde rabies virus vector for high-resolution large-scale reconstruction
of 3D neuron morphology.
Haberl MG(1), Viana da Silva S, Guest JM, Ginger M, Ghanem A, Mulle C,
Oberlaender M, Conzelmann KK, Frick A.
Author information:
(1)Physiopathologie de la plasticité neuronale, INSERM, Neurocentre Magendie,
U862, Bordeaux, France.
Glycoprotein-deleted rabies virus (RABV ∆G) is a powerful tool for the analysis
of neural circuits. Here, we demonstrate the utility of an anterograde RABV ∆G
variant for novel neuroanatomical approaches involving either bulk or sparse
neuronal populations. This technology exploits the unique features of RABV ∆G
vectors, namely autonomous, rapid high-level expression of transgenes, and
limited cytotoxicity. Our vector permits the unambiguous long-range and
fine-scale tracing of the entire axonal arbor of individual neurons throughout
the brain. Notably, this level of labeling can be achieved following infection
with a single viral particle. The vector is effective over a range of ages (>14
months) aiding the studies of neurodegenerative disorders or aging, and infects
numerous cell types in all brain regions tested. Lastly, it can also be readily
combined with retrograde RABV ∆G variants. Together with other modern
technologies, this tool provides new possibilities for the investigation of the
anatomy and physiology of neural circuits.
DOI: 10.1007/s00429-014-0730-z
PMCID: PMC4409643
PMID: 24723034 [Indexed for MEDLINE]