Age-related effects of ethanol consumption on triiodothyronine and retinoic acid nuclear receptors, neurogranin and neuromodulin expression levels in mouse brain.
Neurobiology of Aging. 2006-09-01; 27(9): 1326-1334
DOI: 10.1016/j.neurobiolaging.2005.07.008
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1. Neurobiol Aging. 2006 Sep;27(9):1326-34. Epub 2005 Aug 22.
Age-related effects of ethanol consumption on triiodothyronine and retinoic acid
nuclear receptors, neurogranin and neuromodulin expression levels in mouse brain.
Boucheron C(1), Alfos S, Enderlin V, Husson M, Pallet V, Jaffard R, Higueret P.
Author information:
(1)Unité de Nutrition et Signalisation Cellulaire, EA MENRT; USC INRA, ISTAB,
Université Bordeaux 1, 33405 Talence Cedex, France.
The effects of ethanol consumption and ageing were investigated on the expression
levels of retinoic acid (RA) and triiodothyronine (T3) nuclear receptors (RAR,
RXR and TR) and of associated target genes involved in synaptic plasticity,
neurogranin (RC3) and neuromodulin (GAP-43) in mice brain. For this purpose,
C57BL/6 adult and aged mice were subjected to 5-month ethanol consumption and the
mRNA expression of RAR, RXR, TR, RC3 and GAP-43 was measured using a real-time
RT-PCR method. GAP-43 and RC3 protein levels also were measured by Western blot.
Results showed that 12% ethanol consumption in adult mice (11 months) induced an
increase in RARbeta, RXRbetagamma and TRalphabeta mRNA level in the brain with
only an increase in RC3 expression. The same ethanol consumption in aged mice (22
months) reversed the age-related hypo-expression in brain RARbeta, TRalphabeta
and target genes RC3 and GAP-43. Compared with our previous behavioral data
showing that ethanol is able to partially suppress a selective age-related
cognitive deficit, these results suggest that the ethanol-induced increase in RA
and T3 nuclear receptors expression could be one of the mechanisms involved in
the normalization of synaptic plasticity-associated gene expression altered in
aging brain.
DOI: 10.1016/j.neurobiolaging.2005.07.008
PMID: 16115698 [Indexed for MEDLINE]