A nanoliter-scale nucleic acid processor with parallel architecture
Nat Biotechnol. 2004-03-14; 22(4): 435-439
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1. Nat Biotechnol. 2004 Apr;22(4):435-9. Epub 2004 Mar 14.
A nanoliter-scale nucleic acid processor with parallel architecture.
Hong JW, Studer V, Hang G, Anderson WF, Quake SR.
The purification of nucleic acids from microbial and mammalian cells is a crucial
step in many biological and medical applications. We have developed microfluidic
chips for automated nucleic acid purification from small numbers of bacterial or
mammalian cells. All processes, such as cell isolation, cell lysis, DNA or mRNA
purification, and recovery, were carried out on a single microfluidic chip in
nanoliter volumes without any pre- or postsample treatment. Measurable amounts of
mRNA were extracted in an automated fashion from as little as a single mammalian
cell and recovered from the chip. These microfluidic chips are capable of
processing different samples in parallel, thereby illustrating how highly
parallel microfluidic architectures can be constructed to perform integrated
batch-processing functionalities for biological and medical applications.
PMID: 15024389 [Indexed for MEDLINE]