A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis
Electrophoresis. 2006-08-01; 27(16): 3306-3321
DOI: 10.1002/elps.200500912
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1. Electrophoresis. 2006 Aug;27(16):3306-21.
A complexomic study of Escherichia coli using two-dimensional blue native/SDS
polyacrylamide gel electrophoresis.
Lasserre JP(1), Beyne E, Pyndiah S, Lapaillerie D, Claverol S, Bonneu M.
Author information:
(1)Plateforme Génomique Fonctionnelle Bordeaux – Pôle Protéomique, Université
Victor Segalen Bordeaux 2, Bordeaux, France.
Study of the complexome – all the protein complexes of the cell – is essential
for a better understanding and more global vision of cell function. Using
two-dimensional blue native/SDS-PAGE (2-D BN/SDS-PAGE) technology, the cytosolic
and membrane protein complexes of Escherichia coli were separated. Then, the
different partners of each protein complex were identified by LC-MS/MS. In this
report, 306 protein complexes were separated and identified. Among these protein
complexes, 50 heteromultimeric and 256 homomultimeric protein complexes were
found. Among the 50 heteromultimeric protein complexes, 18 previously described
protein complexes validate the technology. In this study, 109 new protein
complexes were found, providing insight into the function of previously
uncharacterized bacterial proteins.
DOI: 10.1002/elps.200500912
PMID: 16858726 [Indexed for MEDLINE]