3D high- and super-resolution imaging using single-objective SPIM.
Nat Methods. 2015-05-11; 12(7): 641-644
DOI: 10.1038/nmeth.3402
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1. Nat Methods. 2015 Jul;12(7):641-4. doi: 10.1038/nmeth.3402. Epub 2015 May 11.
3D high- and super-resolution imaging using single-objective SPIM.
Galland R(1), Grenci G(2), Aravind A(2), Viasnoff V(3), Studer V(4), Sibarita
JB(4).
Author information:
(1)1] Interdisciplinary Institute for Neuroscience, University of Bordeaux,
Bordeaux, France. [2] Centre National de la Recherche Scientifique, Bordeaux,
France. [3] Mechanobiology Institute, National University of Singapore,
Singapore.
(2)Mechanobiology Institute, National University of Singapore, Singapore.
(3)1] Mechanobiology Institute, National University of Singapore, Singapore. [2]
Bio Mechanics of Cellular Contacts, Centre National de la Recherche Scientifique,
Singapore. [3] Department of Biological Science, National University of
Singapore, Singapore.
(4)1] Interdisciplinary Institute for Neuroscience, University of Bordeaux,
Bordeaux, France. [2] Centre National de la Recherche Scientifique, Bordeaux,
France.
Single-objective selective-plane illumination microscopy (soSPIM) is achieved
with micromirrored cavities combined with a laser beam-steering unit installed on
a standard inverted microscope. The illumination and detection are done through
the same objective. soSPIM can be used with standard sample preparations and
features high background rejection and efficient photon collection, allowing for
3D single-molecule-based super-resolution imaging of whole cells or cell
aggregates. Using larger mirrors enabled us to broaden the capabilities of our
system to image Drosophila embryos.
DOI: 10.1038/nmeth.3402
PMID: 25961414 [Indexed for MEDLINE]