11Beta-Hydroxysteroid Dehydrogenase Messenger Ribonucleic Acid Expression, Bioactivity and Immunoreactivity in Rat Cerebellum.
J Neuroendocrinol. 1990-12-01; 2(6): 853-858
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1. J Neuroendocrinol. 1990 Dec 1;2(6):853-8. doi:
11Beta-Hydroxysteroid Dehydrogenase Messenger Ribonucleic Acid Expression,
Bioactivity and Immunoreactivity in Rat Cerebellum.
Moisan MP(1), Seckl JR, Brett LP, Monder C, Agarwal AK, White PC, Edwards CR.
(1)Department of Medicine, Western General Hospital, Edinburgh EH4 2XU, UK.
Abstract 11beta-Hydroxysteroid dehydrogenase (11beta-OHSD) metabolizes
corticosterone to inactive 11-dehydrocorticosterone and thus protects
non-specific mineralocorticoid receptors from exposure to corticosterone in the
kidney in vivo. Clearly, 11beta-OHSD might also regulate corticosterone access to
glucocorticoid receptors. We have investigated cerebellum, a tissue with high
glucocorticoid receptor, but very low mineralocorticoid receptor levels and have
shown marked 11beta-OHSD bioactivity with similar co-substrate requirements and
inhibition kinetics to the renal enzyme. 11beta-OHSD messenger ribonucleic acid
was expressed in cerebellum and was localized in Purkinje and granule cells. This
distribution was confirmed immunohistochemically. Thus, we provide evidence for
11beta-OHSD in cerebellum and suggest that it may regulate the access of
corticosterone to glucocorticoid receptors in addition to mineralocorticoid