Prospective Intergenerational Consequences of Paternal Stress Experiences on Offspring Immune Health

1. Indian J Orthop. 2025 Oct 8;60(2):392-400. doi: 10.1007/s43465-025-01577-1.
eCollection 2026 Feb.

Assessing the Potential of CD11c as a Biomarker for Identifying Ideal Donors in
Hyaline Cartilage Repair.

Ruksana CA(1), Suresh A(1), Jeyaraj C(2), Priya M(1), Parasuraman G(1), Rebekah
G(3), Vinod E(1)(4).

Author information:
(1)Centre for Stem Cell Research, (A Unit of BRIC-InStem, Bengaluru), Christian
Medical College, Vellore, India.
(2)Department of Orthopaedics, Christian Medical College, Vellore, India.
(3)Department of Biostatistics, Christian Medical College, Vellore, India.
(4)Department of Physiology, Christian Medical College, Tamil Nadu, Vellore,
632002 India.

BACKGROUND: α10β1 integrin (CD11c/CD29) receptor plays a critical role in
anchoring chondrocytes to the extracellular matrix and mediating essential
collagen type II interactions being its primary receptor, thereby influencing
cartilage integrity, matrix synthesis, and organization. Given the challenges
with hyaline cartilage regeneration, particularly the tendency of chondrocytes
to adopt a fibrocartilaginous phenotype due to donor variability and extensive
passage, this study sought to discern differences in chondrogenic potential
between high and low donor groups based on CD11c expression and assess its
potential as a tool for selecting high-potential donors to streamline the
evaluation process.
MATERIALS AND METHODS: Enzymatically isolated chondrocytes from six donors
underwent FACS analysis to determine CD11c expression levels, and based on the
median levels, the chondrocytes were categorized into low and high expression
groups, and their chondrogenic potential was compared using FACS for putative
markers of chondrogenesis, gene expression patterns, differentiation studies and
biochemical analysis of total glycosaminoglycan/DNA ratios and total Collagen
type II levels.
RESULT: High donor group displayed higher variability with CD29 and CD146 but
comparability with the low donor group. Although the high donor group exhibited
lower expression of hypertrophic markers (COL1A1 and RUNX2), suggesting
potential phenotypic advantages, no significant differences were observed in
chondrogenic potential or ECM properties between the two groups.
CONCLUSION: The novel data demonstrate that moderate CD11c percentage expression
with fresh chondrocytes is not a reliable marker for distinguishing donors with
superior chondrogenic potential. Reanalysis using higher percentage expressions
and exploring alternative combinations of markers would help identify optimal
donors.
SUPPLEMENTARY INFORMATION: The online version contains supplementary material
available at 10.1007/s43465-025-01577-1.

© Indian Orthopaedics Association 2025. Springer Nature or its licensor (e.g. a
society or other partner) holds exclusive rights to this article under a
publishing agreement with the author(s) or other rightsholder(s); author
self-archiving of the accepted manuscript version of this article is solely
governed by the terms of such publishing agreement and applicable law.

DOI: 10.1007/s43465-025-01577-1
PMCID: PMC12883622
PMID: 41669227

Conflict of interest statement: Conflict of InterestThe authors declare that
they have no competing interests.

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