Lipid bilayer fluidity and degree of order regulates small EVs adsorption on model cell membrane
Journal of Colloid and Interface Science. 2023-12-01; 652: 1937-1943
DOI: 10.1016/j.jcis.2023.08.117
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https://www.bordeaux-neurocampus.fr/12160
Paba C(1), Dorigo V(2), Senigagliesi B(3), Tormena N(4), Parisse P(5),Voitchovsky K(6), Casalis L(7).
Author information:
(1)Department of Physics, University of Trieste, 34127 Trieste, Italy.
(2)Hochschule Fresenius, 65510 Idstein, Germany.
(3)Elettra Sincrotrone Trieste, 34149 Basovizza TS, Italy.
(4)Department of Physics, University of Durham, Durham DH1 3LE, United Kingdom.
(5)Elettra Sincrotrone Trieste, 34149 Basovizza TS, Italy; IOM-CNR, 34149
Basovizza TS, Italy. Electronic address: .
(6)Department of Physics, University of Durham, Durham DH1 3LE, United Kingdom.
Electronic address: .
(7)Elettra Sincrotrone Trieste, 34149 Basovizza TS, Italy. Electronic address:
.
Small extracellular vesicles (sEVs) are known to play an important role in the
communication between distant cells and to deliver biological information
throughout the body. To date, many studies have focused on the role of sEVs
characteristics such as cell origin, surface composition, and molecular cargo on
the resulting uptake by the recipient cell. Yet, a full understanding of the sEV
fusion process with recipient cells and in particular the role of cell membrane
physical properties on the uptake are still lacking. Here we explore this
problem using sEVs from a cellular model of triple-negative breast cancer fusing
to a range of synthetic planar lipid bilayers both with and without cholesterol,
and designed to mimic the formation of ‘raft’-like nanodomains in cell
membranes. Using time-resolved Atomic Force Microscopy we were able to track the
sEVs interaction with the different model membranes, showing the process to be
strongly dependent on the local membrane fluidity. The strongest interaction and
fusion is observed over the less fluid regions, with sEVs even able to disrupt
ordered domains at sufficiently high cholesterol concentration. Our findings
suggest the biophysical characteristics of recipient cell membranes to be
crucial for sEVs uptake regulation.
Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.
DOI: 10.1016/j.jcis.2023.08.117
PMID: 37690301
Conflict of interest statement: Declaration of Competing Interest The authors
declare that they have no known competing financial interests or personal
relationships that could have appeared to influence the work reported in this
paper.