Image analyser as a tool for the study ofin vitro glomerular vasoreactivity
Cell Biol Toxicol. 1994-12-01; 10(5-6): 291-295
DOI: 10.1007/bf00755772
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1. Cell Biol Toxicol. 1994 Dec;10(5-6):291-5. doi: 10.1007/BF00755772.
Image analyser as a tool for the study of in vitro glomerular vasoreactivity.
Lakhdar B(1), Potier M, L’Azou B, Cambar J.
Author information:
(1)Groupe d’Etude de Physiologie et Physiopathologie Rénales, Faculté de
Pharmacie, Bordeaux, France.
Many drugs used in clinics can dramatically reduce renal hemodynamics. For some
years there have been developed in our laboratory two in vitro glomerular
models, isolated glomeruli and mesangial cell cultures, to quantitate, by video
image analyzer, the direct glomerular effect of vasoreactive agents. The present
study shows the vasoconstrictive effects of angiotensin II and cyclosporin in
both models and compares their glomerular vasoconstriction with or without
vasodilating agents such as verapamil. This drug-induced glomerular
vasoreactivity is time- and dose-dependent; moreover, it can be reversible after
perfusion in control conditions. The interest of these in vitro glomerular
models is validated by fair correlations between in vivo and in vitro data and
between the responses of both. These models can be considered as tools for
assessing glomerular vasoreactivity of nephrotoxic agents.
DOI: 10.1007/BF00755772
PMID: 7697487 [Indexed for MEDLINE]