Inhibition of cyclosporin-induced in vitro glomerular contraction by theophylline
Toxicology in Vitro. 1995-08-01; 9(4): 473-476
DOI: 10.1016/0887-2333(95)00022-z
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1. Toxicol In Vitro. 1995 Aug;9(4):473-6. doi: 10.1016/0887-2333(95)00022-z.
Inhibition of cyclosporin-induced in vitro glomerular contraction by
theophylline.
Potier M(1), Lakhdar B, L’azou B, Cambar J.
Author information:
(1)Laboratoire de Biologie Cellulaire, Faculté de Pharmacie, 3 Place de la
Victoire, 33000, Bordeaux, France.
Cyclosporin A (CsA) induces in vivo severe nephrotoxicity characterized by a
large decrease in renal haemodynamics. The aim of this study is to show the
ability of theophylline, a xanthic derivative, to diminish the CsA-induced
vasoconstrictive effects by using two in vitro rat glomerular models, for
example isolated glomeruli and cultured mesangial cells. Isolated glomeruli are
obtained from the superficial renal cortex of male Sprague-Dawley rats by a
sieving method. Mesangial cells are cultured in RPMI 1640 medium with 15% foetal
calf serum (FCS). The area of either isolated glomeruli or mesangial cells is
assessed by an image analyser with a video camera. Each glomerulus or mesangial
cell serves as its own control by photographing them before any drug incubation
and after incubation for 10, 20 and 30 min either in control solution or control
solution with CsA or CsA and theophylline. CsA (10(-6)m) induces an important
time-dependent decrease in the glomerular area (about 13.4% after 30 min). When
theophylline is added only a slight decrease is noticed (about 3.4% after 30
min). The same results are obtained with mesangial cells. In conclusion, a
direct vasoconstrictive effect of CsA in isolated glomeruli and mesangial cells
can be confirmed. In addition, this effect can be partially prevented by
theophylline.
DOI: 10.1016/0887-2333(95)00022-z
PMID: 20650115