ER-to-Golgi trafficking through a dynamic intermediate cis-Golgi tubular network in Arabidopsis
Nat Cell Biol. 2025-02-25; :
DOI: 10.1038/s41556-025-01624-x
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Fougère L(#)(1), Grison M(#)(1), Laquel P(1), Montrazi M(1), Cordelières F(2), Fernández-Monreal M(2), Poujol C(2), Uemura T(3), Nakano A(4), Ito Y(5), Boutté Y(6).
Author information:
(1)Laboratoire de Biogenèse Membranaire, Université de Bordeaux, CNRS UMR5200,
Villenave d’Ornon, France.
(2)Bordeaux Imaging Center (BIC), Université de Bordeaux, INSERM, CNRS UAR3420,
Bordeaux, France.
(3)Faculty of Core Research, Natural Science Division, Ochanomizu University,
Tokyo, Japan.
(4)Live Cell Super-Resolution Imaging Research Team, RIKEN Center for Advanced
Photonics, Wako, Saitama, Japan.
(5)Institute for Human Life Science, Ochanomizu University, Tokyo, Japan.
(6)Laboratoire de Biogenèse Membranaire, Université de Bordeaux, CNRS UMR5200,
Villenave d’Ornon, France. .
(#)Contributed equally
Endoplasmic reticulum (ER)-to-Golgi trafficking is a central process of the
secretory system of eukaryotic cells that ensures proper spatiotemporal sorting
of proteins and lipids. However, the nature of the ER-Golgi intermediate
compartments (ERGICs) and the molecular mechanisms mediating the transition
between ERGICs and the Golgi, as well as the universality of these processes
among eukaryotes, remain undiscovered. Here we identify a reticulated
tubulo-vesicular network, labelled by MEMBRIN proteins, that is mostly
independent of the Golgi, highly dynamic at the ER-Golgi interface and crossed
by ER-induced released luminal cargos. We find that plant ERGICs become
stabilized by the interaction they establish with pre-existing Golgi and
gradually mature into Golgi cisternae, this process being dependent on
C24-ceramide sphingolipids. Our study is a major twist in the understanding of
the Golgi, as it identifies that the ERGICs in plants comprise a
Golgi-independent and highly dynamic tubular network from which arise more
stable Golgi-associated pre-cisternae structures.
© 2025. The Author(s), under exclusive licence to Springer Nature Limited.
DOI: 10.1038/s41556-025-01624-x
PMID: 40000850
Conflict of interest statement: Competing interests: The authors declare no
competing interests